C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches

The follicle-associated epithelium (FAE) of Peyer's patches (PPs) contains M cells that are important for reducing mucosal immune responses by transporting antigens into the underlying lymphoid tissue. We generated a monoclonal antibody (C6) that reacted with the FAE of calf ileal PPs, and analyzed the characteristics of C6 using immunohistochemistry and Western blotting. FAE of the ileal PP was stained with C6 during both late fetal developmental and postnatal stages. Neither the villous epithelial cell nor intestinal crypt basal cells were stained at any developmental stage. During the prenatal stages, FAE of the jejunal PP was C6-negative. However, a few C6-positive cells were distributed diffusely in some FAE of the jejunal PPs during the postnatal stages. The protein molecular weight of the antigen recognized by C6 was approximately 45 kDa. These data show that C6 is useful for identifying the FAE in ileal PPs and further suggest that differentiation of the FAE in these areas is independent of external antigens.

C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches

The follicle-associated epithelium (FAE) of Peyer's patches (PPs) contains M cells that are important for reducing mucosal immune responses by transporting antigens into the underlying lymphoid tissue. We generated a monoclonal antibody (C6) that reacted with the FAE of calf ileal PPs, and analyzed the characteristics of C6 using immunohistochemistry and Western blotting. FAE of the ileal PP was stained with C6 during both late fetal developmental and postnatal stages. Neither the villous epithelial cell nor intestinal crypt basal cells were stained at any developmental stage. During the prenatal stages, FAE of the jejunal PP was C6-negative. However, a few C6-positive cells were distributed diffusely in some FAE of the jejunal PPs during the postnatal stages. The protein molecular weight of the antigen recognized by C6 was approximately 45 kDa. These data show that C6 is useful for identifying the FAE in ileal PPs and further suggest that differentiation of the FAE in these areas is independent of external antigens.

Growth and histological development of bursa of Fabricius in ducklings / 中国兽医学报

By measuring the absolute weight and growth index of the bursa of Fabricius and using routine H.E method,the growth and histological development of the bursa in 1 to 49 days duckling were studied.The results showed that the absolute weight of the bursa increased as the ducklings grew.The growth index of the bursa reached its peak at 21 days.The height and width of both the large plica and projection formed by the follicle-associated epithelium(FAE)enlarged gradually,the same as the lymphoid follicle area and cortex width.The results indicated that the bursa of Fabricius in duckling develops slowly from 1 to 14 days,and quickly from 14 to 35 days(especially from 21 to 28 days),then steadily from 35 to 49 days.Histologically,the duckling bursa has approached its maturation by the 28th day.

Expression patterns of five kinds of diffuse neuroendocrine system (DNES)cells in duck bursa of Fabricius / 中国兽医学报

The expression patterns of gastrin(Gas),β-endorphin(End).somatostatin(Ss),glucagons(Glu),and 5-hydroxytryptamine (5-HT)cells in the duck bursa of Fabricius were studied by the immunohiswchemicai method associated with image analysis.The resuhs showed that the Gas-positive cells with high intensity wrte seen in the follicle-associated epitllelium (FAE),the End with medium intensity in the interfollicular epithelium,the End,Gas and 5-HT with medium or high intensity in the cortex of lymphoid follicles.and the Gas with high intensity in the medulla of lymphoid follicles.Both SS and Gh showed negative reaction.The pre-sent results demonstrated that the Gas,End and 5-HT cells are differentially expressed in the different parts of the duck bursa.The positive cells distributed in the cortex of lymphoid follicles might favour exerting their influence via an endocrine, autocrine or paracrine pathways on the development of B cells.